1种胞外域仅含1个配体结合区的鸭催乳素受体基因的剪接异形体克隆及基因多态性分析

doi:10.11843/j.issn.0366-6964.2013.05.006

畜牧兽医学报 ›› 2013, Vol. 44 ›› Issue (5): 703-709.doi: 10.11843/j.issn.0366-6964.2013.05.006

1种胞外域仅含1个配体结合区的鸭催乳素受体基因的剪接异形体克隆及基因多态性分析

吉文林^1,2,4，段修军^2,4，宦海琳³，丁潜³，孙国波^2,4，董飚^2,4，陈章言^2,4，陈国宏^1*

（1. 扬州大学动物科学与技术学院，扬州225009； 2. 江苏畜牧兽医职业技术学院，泰州 225300;3. 江苏省农业科学院畜牧研究所，南京 210014； 4. 国家级水禽基因库，泰州 225300）

收稿日期:2012-12-11 出版日期:2013-05-23 发布日期:2013-05-23
通讯作者: 陈国宏，教授，博导，主要从事动物遗传资源评价与利用研究，E-mail: ghchen@yzu.edu.cn
作者简介:吉文林（1958-），男，江苏泰州人，研究员，博士生，主要从事水禽遗传育种研究，E-mail: wenlinj117@126.com
基金资助:
江苏省农业科技自主创新资金项目［cx（10）123］；江苏省农业三新工程项目［SX(2011)198］

An Isoform with Only One Ligand Binding Region of Prolact in Receptor Gene and Its Single Nucleotide Polymorphism in the Duck

JI Wen-lin^1,2,4, DUAN Xiu-jun^2,4, HUAN Hai-lin³, DING Qian³, SUN Guo-bo^2,4,DONG Biao^2,4, CHEN Zhang-yan^2,4, CHEN Guo-hong^1*

(1.College of Animal Science and Technology, Yangzhou University, Yangzhou 225009, China;2.Jiangsu Provincial Animal Husbandry and Veterinary College,Taizhou 225300, China;3. Institute of Animal Science, Jiangsu Academy of Agricultural Sciences, Nanjing 210014,China; 4. National Gene Bank of Waterfowl Resources, Taizhou 225300, China)

Received:2012-12-11 Online:2013-05-23 Published:2013-05-23

摘要/Abstract

摘要：

旨在了解鸭催乳素受体的作用，应用RACE技术克隆到了1种胞外域仅含1个配体结合区的剪接异形体。序列分析表明，剪接异形体长2 535 bp，含431 bp 5′-UTR、1 884 bp编码区和220 bp 3′-UTR，可划分为11个外显子。预测蛋白含627个氨基酸，成熟蛋白603个氨基酸，N端为24个氨基酸信号肽。不同于胞外域串联2个配体结合区的典型禽类催乳素受体，预测蛋白胞外域仅含1个配体结合区，类似于哺乳动物催乳素受体。配体结合区同样含有2对半胱氨酸残基和1个WSXWS的5肽WS基序，此为细胞因子受体超家族I型受体中高度保守的2个典型特征。同时，筛选了催乳素受体基因的1个突变位点和1个SNP位点，分别位于5′-及3′-UTR区。其中，3′-UTR区SNP位点经χ²适合性检验，在鸭群体中未达到Hardy-Weinberg平衡状态。

Abstract:

To understand PRLR’s role in duck, a novel isoform with only one ligand binding region of duck prolactin receptor gene (PRLR) was identified by rapid amplification of cDNA ends (RACE) strategy. Sequence analysis showed that the isoform was 2 535 bp long, contained 431 bp 5′ untranslated region (5′-UTR), 1 884 bp coding sequence and 220 bp 3′ untranslated region (3′-UTR) and comprises 11 exons. The predicted PRLR contained 627 amino acids (aa) and comprises a signal peptide of 24 aa at N-terminus, thus, its mature protein was 603 aa long. Different from canonical avian PRLRs with two tandemly repeated ligand binding regions within the extracellular domain, the putative protein only contained one ligand binding region, similar to the vertebrate PRLRs.The ligand binding region contained 2 pairs of cysteine residues and a pentapeptide of 5 aa known as WS motif as well, the 2 typical features of highly conserved in the members of class 1 cytokine receptor superfamily. In the meanwhile, we investigated one point mutation and one single nucleotide polymorphism (SNP) which located in 5′- and 3′-UTRs of the duck prolactin receptor respectively, and the SNP (C2 391T) in the 3′-UTR was in an extreme departure from Hardy-Weinberg equilibrium in the duck population by Chi-square test.

中图分类号:

S834.2

吉文林，段修军，宦海琳，丁潜，孙国波，董飚，陈章言，陈国宏. 1种胞外域仅含1个配体结合区的鸭催乳素受体基因的剪接异形体克隆及基因多态性分析[J]. 畜牧兽医学报, 2013, 44(5): 703-709.

JI Wen-lin, DUAN Xiu-jun, HUAN Hai-lin, DING Qian, SUN Guo-bo,DONG Biao, CHEN Zhang-yan, CHEN Guo-hong. An Isoform with Only One Ligand Binding Region of Prolact in Receptor Gene and Its Single Nucleotide Polymorphism in the Duck[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2013, 44(5): 703-709.

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参考文献

BEN-JONATHAN N, MERSHON J L, ALLEN D L, et al. Extrapituitary prolactin: distribution, regulation, functions, and clinical aspects ［J］. Endocr Rev, 1996, 17(6):639-669.

GRATTAN D R, KOKAY I C. Prolactin: a pleiotropic neuroendocrine hormone ［J］. J Neuroendocrinol, 2008, 20(6):752-763.

BEN-JONATHAN N, LAPENSEE C R, LAPENSEEE E W. What can we learn from rodents about prolactin in humans? ［J］.Endocr Rev, 2008, 29(1):1-41.

BACHELOT A, BINART N. Reproductive role of prolactin［J］. Reproduction, 2007, 133(2):361-369.

FREEMAN M E, KANYICSKA B, LERANT A, et al. Prolactin: structure, function, and regulation of secretion ［J］. Physiol Rev, 2000, 80(4):1523-1631.

BOKE-FEYSOT C, GOFFIN V, EDERY M, et al. Prolactin (PRL) and its receptor: actions, signal transduction pathways and phenotypes observed in PRL receptor knockout mice ［J］. Endocr Rev, 1998, 19(3):225-268.

CLARKE D L, LINZER D I. Changes in prolactin receptor expression during pregnancy in the mouse ovary ［J］. Endocrinology, 1993, 133(1): 224-232.

SHIROTA M, BANVILLE D, ALI S. Expression of two forms of prolactin receptor in rat ovary and liver ［J］. Mol Endocrinol, 1990, 4(8):1136-1143.

BOUTIN J M, EDERY M, SHIROTA M, et al. Identification of a cDNA encoding a long form of prolactin receptor in human hepatoma and breast cancer cells ［J］. Mol Endocrinol, 1989, 3: 1455-1461.

TOMAS A, CASELLAS J, RAMIREZ O, et al. High amino acid variation in the intracellular domain of the pig prolactin receptor (PRLR) and its relation to ovulation rate and piglet survival traits ［J］. J Anim Sci, 2006, 84(8): 1991-1998.

SCOTT P, KESSLE M A, SCHULER L A. Molecular cloning of the bovine prolactin receptor and distribution of prolactin and growth hormone receptor transcript in fetal and utero-placental tissues ［J］. Mol Cell Endocrinol, 1992, 89: 47-58.

BIGNON C, BINART N, ORMANDY C, et al. Long and short forms of the ovine prolactin receptor: cDNA cloning and genomic analysis reveal that the two forms arise by different alternative splicing mechanisms in ruminants and in rodents ［J］. J Mol Endocrinol, 1997, 19(2):109-120.

EDERY M, JOLICOEUR C, LEVI-MEYRUEIS C, et al. Identification and sequence analysis of a second form of prolactin receptor by molecular cloning of complementary DNA from rabbit mammary gland ［J］. Proc Natl Acad Sci USA, 1989, 86: 2112-2116.

TANAKA M, MAEDA K, OKUBO T, et al. Double antenna structure of chicken prolactin receptor deduced from the cDNA sequence ［J］. Biochem Biophys Res Commun, 1992, 188(2): 490-496.

WANG J, HOU S S, HUANG W, et al. Molecular cloning of prolactin receptor of the Peking duck ［J］. Poult Sci, 2009, 88(5):1016-1022.

XING G, ZHAO Q, MAO J, et al. Identification and characterization of goose prolactin receptor ［J］. Poult Sci, 2011, 90(5):1050-1057.

ZHOU J F, ZADWORNY D, GUEMENE D, et al. Molecular cloning, tissue distribution, and expression of the prolactin receptor during various reproductive states in Meleagris gallopavo ［J］. Biol Reprod,1996, 55(5): 1081-1090.

LU A, HU X, CHEN H, et al. Single nucleotide polymorphisms of the prolactin receptor (PRLR) gene and its association with growth traits in Chinese cattle ［J］. Mol Biol Rep, 2011, 38(1):261-266.

LU A, HU X, CHEN H, et al. Novel SNPs of the bovine PRLR gene associated with milk production traits ［J］. Biochem Genet, 2011, 49(3-4):177-189.

SMIRNOVA O V, BOGORAD R L. Short forms of membrane receptors: generation and role in hormonal signal transduction ［J］. Biochemistry (Mosc), 2004, 69(4):351-363.

NOGAMI H, HOSHINO R, OGASAWARA K, et al. Region-specific expression and hormonal regulation of the first exon variants of rat prolactin receptor mRNA in rat brain and anterior pituitary gland ［J］. J Neuroendocrinol, 2007, 19(8):583-593.

ORMANDY C J, BINART N, HELLOCO C, et al. Mouse prolactin receptor gene: genomic organization reveals alternative promoter usage and generation of isoforms via alternative 3′-exon splicing ［J］. DNA Cell Biol,1998, 17(9):761-770.

TROTT J F, HOVERY R C, KODURI S, et al. Alternative splicing to exon 11 of human prolactin receptor gene results in multiple isoforms including a secreted prolactin-binding protein ［J］. J Mol Endocrinol, 2003, 30(1):31-47.

KLINE J B, RYCYZYN M A, CLEVENGER C V. Characterization of a novel and functional human prolactin receptor isoform (deltaS1PRLr) containing only one extracellular fibronectin-like domain ［J］. Mol Endocrinol, 2002, 16(10):2310-2322.

BRUNSTEIN J, SODERLUNDVENERMO M, HEDMAN K. Identification of a novel RNA splicing pattern as a basis of restricted cell tropism of erythrovirus B19 ［J］. Virology, 2000, 274(2):284-291.

NIXON J E, WANG A, MORRISON H G, et al. A spliceosomal intron in Giardia lamblia ［J］. Proc Natl Acad Sci USA, 2002, 99(6):3701-3705.

SHASTRY B S. SNPs: impact on gene function and phenotype ［J］. Methods Mol Biol, 2009, 578:3-22.

贾汝敏,吴慧英,刘铀,等.狮头鹅PRL基因的克隆及原核表达［J］.畜牧兽医学报,2011, 42(3): 343-348.

HALUSHKA M K, FAN J B, BENTLEY K, et al. Patterns of singlenucleotide polymorphisms in candidate genes for blood-pressure homeostasis ［J］. Nat Genet, 1999, 22(3):239-247.

SHASTRY B S. SNPs: impact on gene function and phenotype ［J］. Methods Mol Biol, 2009,578:3-22.

束婧婷,李慧芳,邹剑敏,等.基于连接酶检测反应的PRL基因多态与清远麻鸡繁殖性能的相关性研究［J］.畜牧兽医学报,2011, 42(8): 1095-1100.

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1种胞外域仅含1个配体结合区的鸭催乳素受体基因的剪接异形体克隆及基因多态性分析

An Isoform with Only One Ligand Binding Region of Prolact in Receptor Gene and Its Single Nucleotide Polymorphism in the Duck

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